Journal: Antibodies

Article Title: Tuning Relative Polypeptide Expression to Optimize Assembly, Yield and Downstream Processing of Bispecific Antibodies

doi: 10.3390/antib7030029

Figure Lengend Snippet: IgG expression of K2O30, K2O35, K2O41 and their respective codon de-optimized variants after transfection in Transformed Human Embryo Kidney monolayer epithelial cells (PEAK cells). ( A ) Electrophoresis using the Agilent Bioanalyzer 2100 for total IgG obtained after the first affinity chromatography step capturing all three IgG forms. The bands corresponding to the common heavy chain (HC), the lambda light chain (λ LC) and the kappa light chain (κ LC) in reducing and denaturing conditions are indicated. ( B ) Ratio of the light chains as determined from the band intensity of the Agilent result. ( C ) Total IgG concentration in the culture supernatant.

Article Snippet: Transformed Human Embryo Kidney monolayer epithelial cells (PEAK cells; Edge Bio, La Jolla, CA, USA) were maintained in 5% CO 2 at 37 °C in a humidified atmosphere in DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% FCS (Sigma-Aldrich, St Louis, MO, USA) and supplemented with 2 mM glutamine (Sigma-Aldrich, St Louis, MO, USA), referring to complete DMEM.

Techniques: Expressing, Transfection, Transformation Assay, Electrophoresis, Affinity Chromatography, Concentration Assay